明天给大家分享一篇文献，看看作者是如何经过数据发掘，发现癌症治疗的靶标？
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期刊引见
明天要引见的这篇文章是我们中国人发表在ONCOLOGY REPORTS上的文章，ONCOLOGY REPORTS 2017年的影响因子为3.04，算是3分的文章。
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文章标题
Bioinformatic analysis suggests that UGT2B15 activates the Hippo‑YAP signaling pathway leading to the pathogenesis of gastric cancer
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分析内容
首先，第一步，作者在GEO数据库上下载了三个研讨的数据：GSE54129，GSE79973，GSE56807，然后经过GEO数据库自带的工具GEO2R做差异表达分析，然后取交集的差异基因。
第二步，做GO富集分析
第三步，KEGG富集分析
第四步，PPI分析以及挑选hub基因
第五步，查看hub基因在各种条件的表达程度
第六步，停止生活分析
第七步，停止免疫组化检验
最后，作者得住结论：UGT2B15能作为癌症治疗的靶标。
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文章摘要
附录原文的摘要：
Gastric cancer (GC) is one of the most common
malignancies that threatens human health. As the molecular
mechanisms unerlying GC are not completely understood,
identification of genes related to GC could provide new insights
into gene function as well as potential treatment targets. We
discovered that UGT2B15 may contribute to the pathogenesis
and progression of GC using GEO data and bioinformatic
analysis. Using TCGA data, UGT2B15 mRNA was found to be
significantly overexpressed in GC tissues; patients with higher
UGT2B15 had a poorer prognosis. It was further discovered that
UGT2B15 and FOXA1 were both upregulated, and UGT2B15
and Foxa1 were positively correlated in GC. It is known that
Foxa1 is a vital threshold to activate the Hippo‑YAP signaling
pathway. In addition, we suggest that a potential molecular
mechanisms includes UGT2B15 which may upregulate Foxa1,
activate the Hippo‑YAP signaling pathway and contribute to the
development of GC. Taken together, our findings demonstrate
that UGT2B15 may be an oncogene in GC and is a promising
therapeutic target for cancer treatment. |
